GSM5661561: KOD1-WT-Total-RNA-sample; Thermococcus kodakarensis KOD1;... - SRA

SRX12834643: GSM5661561: KOD1-WT-Total-RNA-sample; Thermococcus kodakarensis KOD1; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 101.4M spots, 10.3G bases, 5.8Gb downloads

Submitted by: NCBI (GEO)

Study: Genetic and functional analysis of archaeal ATP-dependent RNA ligase in ribosomal RNA biogenesis

PRJNA776174 • SRP343732 • All experiments • All runs

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In this study, we generated allelic knockouts of ATP-dependent RNA ligase (Rnl) in hyperthermophilic archaeon T. kodakarensis and analyze the total and small RNA by RNA-Seq. Overall design: Knocked out the TK1545 gene by homologous recombination, and prepared RNA samples for whole transcriptosome and small RNA seq analysis. The differentially expressed genes between the two strains were analyzed.

Sample: KOD1-WT-Total-RNA-sample

SAMN22771672 • SRS10779478 • All experiments • All runs

Organism: Thermococcus kodakarensis KOD1

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: T. kodakarensis total RNA was isolated from WT and tk1545KO cultures using TRIZOL reagent (Invitrogen) RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM5661561

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 101.4M spots, 10.3G bases, 5.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR16633624	101,376,053	10.3G	5.8Gb	2021-11-02

ID:: 17493711

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